Mutations in ORP1 Conferring Oxathiapiprolin Resistance Confirmed by Genome Editing using CRISPR/Cas9 in Phytophthora capsici and P. sojae.
Identifieur interne : 000716 ( Main/Exploration ); précédent : 000715; suivant : 000717Mutations in ORP1 Conferring Oxathiapiprolin Resistance Confirmed by Genome Editing using CRISPR/Cas9 in Phytophthora capsici and P. sojae.
Auteurs : Jianqiang Miao ; Yuandong Chi ; Dong Lin ; Brett M. Tyler ; Xili LiuSource :
- Phytopathology [ 0031-949X ] ; 2018.
Descripteurs français
- KwdFr :
- Capsicum (microbiologie), Fongicides industriels (pharmacologie), Hydrocarbures fluorés (pharmacologie), Maladies des plantes (microbiologie), Mutation ponctuelle (MeSH), Phytophthora (effets des médicaments et des substances chimiques), Phytophthora (génétique), Phénotype (MeSH), Pyrazoles (pharmacologie), Récepteurs aux stéroïdes (génétique), Résistance aux substances (génétique), Sporanges (effets des médicaments et des substances chimiques), Sporanges (génétique), Systèmes CRISPR-Cas (MeSH), Édition de gène (MeSH).
- MESH :
- effets des médicaments et des substances chimiques : Phytophthora, Sporanges.
- génétique : Phytophthora, Récepteurs aux stéroïdes, Résistance aux substances, Sporanges.
- microbiologie : Capsicum, Maladies des plantes.
- pharmacologie : Fongicides industriels, Hydrocarbures fluorés, Pyrazoles.
- Mutation ponctuelle, Phénotype, Systèmes CRISPR-Cas, Édition de gène.
English descriptors
- KwdEn :
- CRISPR-Cas Systems (MeSH), Capsicum (microbiology), Drug Resistance (genetics), Fungicides, Industrial (pharmacology), Gene Editing (MeSH), Hydrocarbons, Fluorinated (pharmacology), Phenotype (MeSH), Phytophthora (drug effects), Phytophthora (genetics), Plant Diseases (microbiology), Point Mutation (MeSH), Pyrazoles (pharmacology), Receptors, Steroid (genetics), Sporangia (drug effects), Sporangia (genetics).
- MESH :
- chemical , genetics : Receptors, Steroid.
- chemical , pharmacology : Fungicides, Industrial, Hydrocarbons, Fluorinated, Pyrazoles.
- drug effects : Phytophthora, Sporangia.
- genetics : Drug Resistance, Phytophthora, Sporangia.
- microbiology : Capsicum, Plant Diseases.
- CRISPR-Cas Systems, Gene Editing, Phenotype, Point Mutation.
Abstract
Oxathiapiprolin is a novel fungicide that was recently registered in a number of countries to control plant-pathogenic oomycetes such as Phytophthora capsici. In our previous study, point mutations G770V and G839W in oxysterol binding protein-related protein 1 (ORP1) were detected in oxathiapiprolin-resistant P. capsici isolates (PcORP1). Here, we used the CRISPR/Cas9 system to verify the effects of these two point mutations on P. capsici phenotypes. Transformants containing heterozygous G770V and G839W mutations in PcORP1 showed high levels of oxathiapiprolin resistance. The G770V transformants showed otherwise similar phenotypes compared with the wild-type isolate BYA5, including sporangia and zoospore production, cyst germination, and pathogenicity. However, two independent transformants with heterozygous G839W mutations in PcORP1 could not produce sporangia. Three transformants with an unexpected point mutation in PcORP1 (ΔN837) showed high oxathiapiprolin resistance, and either similar or significantly reduced fitness compared with BYA5. The same deletion (ΔN837) was confirmed to confer oxathiapiprolin resistance in P. sojae by using CRISPR/Cas9. These homozygous P. sojae mutants also showed either similar or strongly reduced fitness compared with the wild-type parent isolate P6497. These results improve our understanding of oxathiapiprolin resistance in Phytophthora spp., and will be useful for the development of novel oxysterol-binding protein homolog inhibitor fungicides.
DOI: 10.1094/PHYTO-01-18-0010-R
PubMed: 29979095
Affiliations:
Links toward previous steps (curation, corpus...)
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Tyler</name><affiliation><nlm:affiliation>First and fifth authors: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling 712100, China; first, second, third, and fifth authors: Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China; and first and fourth authors: Center for Genome Research and Biocomputing and Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331.</nlm:affiliation><wicri:noCountry code="subField">Corvallis 97331</wicri:noCountry></affiliation></author><author><name sortKey="Liu, Xili" sort="Liu, Xili" uniqKey="Liu X" first="Xili" last="Liu">Xili Liu</name><affiliation><nlm:affiliation>First and fifth authors: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling 712100, China; first, second, third, and fifth authors: Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China; and first and fourth authors: Center for Genome Research and Biocomputing and Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331.</nlm:affiliation><wicri:noCountry code="subField">Corvallis 97331</wicri:noCountry></affiliation></author></analytic><series><title level="j">Phytopathology</title><idno type="ISSN">0031-949X</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>CRISPR-Cas Systems (MeSH)</term><term>Capsicum (microbiology)</term><term>Drug Resistance (genetics)</term><term>Fungicides, Industrial (pharmacology)</term><term>Gene Editing (MeSH)</term><term>Hydrocarbons, Fluorinated (pharmacology)</term><term>Phenotype (MeSH)</term><term>Phytophthora (drug effects)</term><term>Phytophthora (genetics)</term><term>Plant Diseases (microbiology)</term><term>Point Mutation (MeSH)</term><term>Pyrazoles (pharmacology)</term><term>Receptors, Steroid (genetics)</term><term>Sporangia (drug effects)</term><term>Sporangia (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Capsicum (microbiologie)</term><term>Fongicides industriels (pharmacologie)</term><term>Hydrocarbures fluorés (pharmacologie)</term><term>Maladies des plantes (microbiologie)</term><term>Mutation ponctuelle (MeSH)</term><term>Phytophthora (effets des médicaments et des substances chimiques)</term><term>Phytophthora (génétique)</term><term>Phénotype (MeSH)</term><term>Pyrazoles (pharmacologie)</term><term>Récepteurs aux stéroïdes (génétique)</term><term>Résistance aux substances (génétique)</term><term>Sporanges (effets des médicaments et des substances chimiques)</term><term>Sporanges (génétique)</term><term>Systèmes CRISPR-Cas (MeSH)</term><term>Édition de gène (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Receptors, Steroid</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Fungicides, Industrial</term><term>Hydrocarbons, Fluorinated</term><term>Pyrazoles</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Phytophthora</term><term>Sporangia</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Phytophthora</term><term>Sporanges</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Drug Resistance</term><term>Phytophthora</term><term>Sporangia</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Phytophthora</term><term>Récepteurs aux stéroïdes</term><term>Résistance aux substances</term><term>Sporanges</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Capsicum</term><term>Maladies des plantes</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Capsicum</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Fongicides industriels</term><term>Hydrocarbures fluorés</term><term>Pyrazoles</term></keywords><keywords scheme="MESH" xml:lang="en"><term>CRISPR-Cas Systems</term><term>Gene Editing</term><term>Phenotype</term><term>Point Mutation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Mutation ponctuelle</term><term>Phénotype</term><term>Systèmes CRISPR-Cas</term><term>Édition de gène</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Oxathiapiprolin is a novel fungicide that was recently registered in a number of countries to control plant-pathogenic oomycetes such as Phytophthora capsici. In our previous study, point mutations G770V and G839W in oxysterol binding protein-related protein 1 (ORP1) were detected in oxathiapiprolin-resistant P. capsici isolates (PcORP1). Here, we used the CRISPR/Cas9 system to verify the effects of these two point mutations on P. capsici phenotypes. Transformants containing heterozygous G770V and G839W mutations in PcORP1 showed high levels of oxathiapiprolin resistance. The G770V transformants showed otherwise similar phenotypes compared with the wild-type isolate BYA5, including sporangia and zoospore production, cyst germination, and pathogenicity. However, two independent transformants with heterozygous G839W mutations in PcORP1 could not produce sporangia. Three transformants with an unexpected point mutation in PcORP1 (ΔN837) showed high oxathiapiprolin resistance, and either similar or significantly reduced fitness compared with BYA5. The same deletion (ΔN837) was confirmed to confer oxathiapiprolin resistance in P. sojae by using CRISPR/Cas9. These homozygous P. sojae mutants also showed either similar or strongly reduced fitness compared with the wild-type parent isolate P6497. These results improve our understanding of oxathiapiprolin resistance in Phytophthora spp., and will be useful for the development of novel oxysterol-binding protein homolog inhibitor fungicides.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29979095</PMID><DateCompleted><Year>2019</Year><Month>02</Month><Day>14</Day></DateCompleted><DateRevised><Year>2019</Year><Month>02</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0031-949X</ISSN><JournalIssue CitedMedium="Print"><Volume>108</Volume><Issue>12</Issue><PubDate><Year>2018</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Phytopathology</Title><ISOAbbreviation>Phytopathology</ISOAbbreviation></Journal><ArticleTitle>Mutations in ORP1 Conferring Oxathiapiprolin Resistance Confirmed by Genome Editing using CRISPR/Cas9 in Phytophthora capsici and P. sojae.</ArticleTitle><Pagination><MedlinePgn>1412-1419</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/PHYTO-01-18-0010-R</ELocationID><Abstract><AbstractText>Oxathiapiprolin is a novel fungicide that was recently registered in a number of countries to control plant-pathogenic oomycetes such as Phytophthora capsici. In our previous study, point mutations G770V and G839W in oxysterol binding protein-related protein 1 (ORP1) were detected in oxathiapiprolin-resistant P. capsici isolates (PcORP1). Here, we used the CRISPR/Cas9 system to verify the effects of these two point mutations on P. capsici phenotypes. Transformants containing heterozygous G770V and G839W mutations in PcORP1 showed high levels of oxathiapiprolin resistance. The G770V transformants showed otherwise similar phenotypes compared with the wild-type isolate BYA5, including sporangia and zoospore production, cyst germination, and pathogenicity. However, two independent transformants with heterozygous G839W mutations in PcORP1 could not produce sporangia. Three transformants with an unexpected point mutation in PcORP1 (ΔN837) showed high oxathiapiprolin resistance, and either similar or significantly reduced fitness compared with BYA5. The same deletion (ΔN837) was confirmed to confer oxathiapiprolin resistance in P. sojae by using CRISPR/Cas9. These homozygous P. sojae mutants also showed either similar or strongly reduced fitness compared with the wild-type parent isolate P6497. These results improve our understanding of oxathiapiprolin resistance in Phytophthora spp., and will be useful for the development of novel oxysterol-binding protein homolog inhibitor fungicides.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Miao</LastName><ForeName>Jianqiang</ForeName><Initials>J</Initials><Identifier Source="ORCID">0000-0002-4683-6933</Identifier><AffiliationInfo><Affiliation>First and fifth authors: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling 712100, China; first, second, third, and fifth authors: Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China; and first and fourth authors: Center for Genome Research and Biocomputing and Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chi</LastName><ForeName>Yuandong</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>First and fifth authors: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling 712100, China; first, second, third, and fifth authors: Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China; and first and fourth authors: Center for Genome Research and Biocomputing and Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Dong</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>First and fifth authors: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling 712100, China; first, second, third, and fifth authors: Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China; and first and fourth authors: Center for Genome Research and Biocomputing and Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tyler</LastName><ForeName>Brett M</ForeName><Initials>BM</Initials><AffiliationInfo><Affiliation>First and fifth authors: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling 712100, China; first, second, third, and fifth authors: Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China; and first and fourth authors: Center for Genome Research and Biocomputing and Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Xili</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>First and fifth authors: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling 712100, China; first, second, third, and fifth authors: Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China; and first and fourth authors: Center for Genome Research and Biocomputing and Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>10</Month><Day>31</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Phytopathology</MedlineTA><NlmUniqueID>9427222</NlmUniqueID><ISSNLinking>0031-949X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005659">Fungicides, Industrial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006845">Hydrocarbons, Fluorinated</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011720">Pyrazoles</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011987">Receptors, Steroid</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C054899">oxysterol binding protein</NameOfSubstance></Chemical><Chemical><RegistryNumber>56498AIV8R</RegistryNumber><NameOfSubstance UI="C000592431">oxathiapiprolin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D064113" MajorTopicYN="N">CRISPR-Cas Systems</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002212" MajorTopicYN="N">Capsicum</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004351" MajorTopicYN="N">Drug Resistance</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005659" MajorTopicYN="N">Fungicides, Industrial</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000072669" MajorTopicYN="N">Gene Editing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006845" MajorTopicYN="N">Hydrocarbons, Fluorinated</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017354" MajorTopicYN="N">Point Mutation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011720" MajorTopicYN="N">Pyrazoles</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011987" MajorTopicYN="N">Receptors, Steroid</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D058432" MajorTopicYN="N">Sporangia</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>7</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>2</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>7</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29979095</ArticleId><ArticleId IdType="doi">10.1094/PHYTO-01-18-0010-R</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list></list><tree><noCountry><name sortKey="Chi, Yuandong" sort="Chi, Yuandong" uniqKey="Chi Y" first="Yuandong" last="Chi">Yuandong Chi</name><name sortKey="Lin, Dong" sort="Lin, Dong" uniqKey="Lin D" first="Dong" last="Lin">Dong Lin</name><name sortKey="Liu, Xili" sort="Liu, Xili" uniqKey="Liu X" first="Xili" last="Liu">Xili Liu</name><name sortKey="Miao, Jianqiang" sort="Miao, Jianqiang" uniqKey="Miao J" first="Jianqiang" last="Miao">Jianqiang Miao</name><name sortKey="Tyler, Brett M" sort="Tyler, Brett M" uniqKey="Tyler B" first="Brett M" last="Tyler">Brett M. 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